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| KRAUSKOPF,MANUEL. |
| During the last decade the articles published by Chilean Research Centers grew 1,73 which compares to the 2.34 fold increase of mainstream research articles registered as a whole in Latin America. However, the relative impact of the Chilean publications surpassed that of Latin America. In Biological Sciences, traditionally the strongest research area within Chile, Latin America also shows a steeper slope of growth. Qualitatively, biological disciplines in Chile are comparable to those published in Latin America although in Chile there are specialties as Physiology that surpass the average world's impact. The scientometric data is consistent with the fall in individual grants that the Chilean Research Fund (FONDECYT) has been allocating during the last... |
| Tipo: Journal article |
Palavras-chave: Research output; Biological sciences; Chile; Latin America; Scientometric analyses. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100012 |
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| Gusso,Aníbal; Ducati,Jorge Ricardo; Cotlier,Carlos G; Lopez,Diego A. G. |
| A search is made for indicators of the presence of phytoplankton, using satellite images of the Pacific (Central Chile) and Atlantic (South Brazil). AVHRR/NOAA-16 and -17 visible (1 and 2) and thermal (4 and 5) channels were used to perform a detection test, respectively, the Suspended Particulate Matter (SPM) and the Sea Surface Temperature (SST). In Brazilian waters, a positive correlation is found between SST and SPM's reflectance. This is interpreted as due to phytoplankton being more abundant in colder waters, where nutrients availability are higher because CO2 dissolution rates, thus being a favorable environment for phytoplankton contents, which when mixed with SPM, tends to reduce the total water reflectance, since organic matter causes absorption... |
| Tipo: Journal article |
Palavras-chave: Phytoplankton biomass; Remote sensing; AVHRR/NOAA. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382004000200046 |
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| ARANDA,EVELYN; OWEN,GARETH I. |
| Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in matrigel. This valid and simple method has the drawbacks that each cell population is distinct and the constraint of obtaining primary source material. Herein we utilize the established EA.hy926 endothelial cell line as our model for in vitro angiogenesis and present a novel formula to quantify endothelial cell remodeling to identify pro- and anti-angiogenic agents. Furthermore, our technique details the procedures to identify and quantify compounds that have... |
| Tipo: Journal article |
Palavras-chave: Angiogenesis; EA.hy926; HUVEC; Endothelial; Formula; In vitro assay; Matrigel. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300012 |
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| Sun,Xuerong; Liu,Xinguang; Zhang,Yuehong; Kuang,Xielan; Lv,Bo; Ge,Jian. |
| Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is... |
| Tipo: Journal article |
Palavras-chave: Mechanical pressure; Cell culture; Transwell culture system; Cytoskeleton. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007 |
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| Chen,Sique; Zheng,Xiujuan; Cao,Hongrui; Jiang,Linghui; Liu,Fangqian; Sun,Xinli. |
| Background Thermostable DNA polymerase (Taq Pol ?) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method. The method used ammonium sulfate to precipitate the enzyme, and it saved effort and money but not time. Moreover, we found that 30-40% activity of Taq Pol I was lost at the ammonium sulfate precipitation step, and the product contained a small amount of DNA. Results We provided a novel, simplified and low-cost method to purify the Taq Pol ? after overproduction of the enzyme in Escherichia coli, which used ethanol instead of ammonium sulfate to precipitate the enzyme. The precipitate can be directly dissolved in the storage buffer without dialysis. In addition, DNA and RNA contamination was removed with... |
| Tipo: Journal article |
Palavras-chave: Ethanol precipitation; PCR; Purification; Taq DNA polymerase. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500005 |
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| Zhang,Fanfan; Ye,Yu; Song,Deping; Guo,Nannan; Peng,Qi; Li,Anqi; Zhou,Xingrong; Chen,Yanjun; Zhang,Min; Huang,Dongyan; Tang,Yuxin. |
| Abstract Background Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical samples is urgently needed. Results In this study, we developed a single-tube one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay specific for nucleocapsid gene to diagnose and monitor PDCoV infections. The detection limit of RT-LAMP assay was 1 × 101 copies of PDCoV, which was approximately 100-fold more sensitive than gel-based one-step reverse transcription polymerase chain... |
| Tipo: Journal article |
Palavras-chave: Porcine Deltacoronavirus (PDCoV); RT-LAMP; Rapid diagnosis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100222 |
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| Morales-Salinas,Luis; Acevedo,Edmundo; Castellaro,Giorgio; Román-Osorio,Luis; Morales-Inostroza,Jael; Alonso,Máximo F. |
| In the past 20 years, different areas of research concerning native and exotic species, herbaceous crops and forest plantations have been oriented toward satisfying domestic, industrial and transportation energy requirements. Because bioenergy species constitute an important resource, it would be strategic for a country to have a method for identifying areas suitable for their cultivation to properly incorporate the establishment of energy crops into land use planning. In this study, we sought to define the suitable territories for 16 bioenergy species and their energy potential based on their soil and climate requirements in Central and Southern Chile. We used an adapted version of the FAO EcoCrop database implemented through DIVA-GIS software to predict... |
| Tipo: Journal article |
Palavras-chave: Bioenergy species; EcoCrop; Ecological niche model; Land suitability. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000200009 |
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| Zhao,Yongliang; Dong,Zongming; Li,Tengfei; Huang,Gang. |
| Background: The AdEasy system is a fast-track system for generating recombinant adenoviruses using the efficient homologous recombination machinery between shuttle and adenovirus backbone plasmids in Escherichia coli BJ5183 cells. The key step is homologous recombination in BJ5183 cells, which is driven by RecA activity. However, culture time is stringently limited to reduce the damage to recombinant plasmids by RecA activity. Therefore, rapid identification of recombinant adenoviruses within the limited time-period is critical. Results: We developed a simple negative selection method to identify recombinant adenoviruses using colony PCR, which improves the efficiency of adenovirus recombination screening and packaging. Conclusions: The negative selection... |
| Tipo: Journal article |
Palavras-chave: Adenovirus; AdEasy; Colony PCR; Homologous recombination; RecA. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100008 |
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| Castellaro G,Giorgio; Aguilar G,Claudio; Vera I,Raul; Morales S,Luis. |
| Grasslands are complex ecosystems and their processes are affected by soil, meteorological, and management variables. In this context, dynamic simulation models are useful to understand these processes and to design grassland use strategies. The aim of this study was to develop and validate a simulation model of perennial pasture growth based on soil and climate variables. A first approach considered that soil fertility levels were adequate; therefore, soil water availability and phytomass level were the main variables affecting pasture growth. The subroutines considered were water balance, pasture growth, and root biomass dynamics. The hypotheses regarding the functioning of the system were formulated as a group of equations which were solved numerically... |
| Tipo: Journal article |
Palavras-chave: Grassland simulation models; Mesophytic grasslands. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000300013 |
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| la Jara,Jorge Jimenez de; Bastias,Gabriel; Ferreccio,Catterina; Moscoso,Cristian; Sagues,Sofia; Cid,Camilo; Bronstein,Eduardo; Herrera,Cristian; Nervi,Bruno; Corvalan,Alejandro; Velasquez,Ethel V; Gonzalez,Pamela; Castellon,Enrique; Bustamante,Eva; Oñate,Sergio; McNerney,Eileen; Sullivan,Richard; Owen,Gareth I. |
| INTRODUCTION: The South American country Chile now boasts a life expectancy of over 80 years. As a consequence, Chile now faces the increasing social and economic burden of cancer and must implement political policy to deliver equitable cancer care. Hindering the development of a national cancer policy is the lack of comprehensive analysis of cancer infrastructure and economic impact. OBJECTIVES: Evaluate existing cancer policy, the extent of national investigation and the socio-economic impact of cancer to deliver guidelines for the framing of an equitable national cancer policy. METHODS: Burden, research and care-policy systems were assessed by triangulating objective system metrics -epidemiological, economic, etc. - with political and policy analysis.... |
| Tipo: Journal article |
Palavras-chave: Chile; Cancer policy; Investigation; Research and development; Statistics; Gallbladder cancer; Stomach cancer; Developing country. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100010 |
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| Seo,Hyang‑Hee; Kim,Sang Woo; Lee,Chang Youn; Lim,Kyu Hee; Lee,Jiyun; Choi,Eunhyun; Lim,Soyeon; Lee,Seahyoung; Hwang,Ki‑Chul. |
| Abstract Background Pathologic vascular smooth muscle cell (VSMC) proliferation and migration after vascular injury promotes the development of occlusive vascular disease. Therefore, an effective chemical agent to suppress aberrant proliferation and migration of VSMCs can be a potential therapeutic modality for occlusive vascular disease such as atherosclerosis and restenosis. To find an anti-proliferative chemical agent for VSMCs, we screened an in-house small molecule library, and the selected small molecule was further validated for its anti-proliferative effect on VSMCs using multiple approaches, such as cell proliferation assays, wound healing assays, transwell migration assays, and ex vivo aortic ring assay. Results Among 43 initially screened... |
| Tipo: Journal article |
Palavras-chave: Syk kinase inhibitor; BAY61-3606 VSMC; Proliferation; Migration. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100403 |
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| Sarwat,Maryam; Singh Negi,Madan; Lakshmikumaran,Malathi; Kumar Tyagi,Akhilesh; Das,Sandip; Shankar Srivastava,Prem. |
| For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). |
| Tipo: Journal article |
Palavras-chave: DNA extraction; Medicinal plants; Molecular markers; Terminalia species. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100011 |
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| Fuentealba,Claudia; Pedreschi,Romina; Hernández,Ignacia; Saavedra,Jorge. |
| C. Fuentealba, R. Pedreschi, I. Hernández, and J. Saavedra. 2016. A statistical approach for assessing the heterogeneity of Hass avocados subjected to different postharvest abiotic stresses. Cien. Inv. Agr. 43(3):356-365. Hass avocado (Persea americana Mill.) is marketed worldwide. Due to its complex physiology, a wide variability in postharvest ripening can be observed, i.e., heterogeneity in the number of days to reach edible ripeness. There is a need for a simple and objective method for evaluating the efficacy of postharvest treatments in the reduction of this postharvest ripening heterogeneity given the high demand of import countries for consistent quality and product homogeneity. Therefore, the aim of this study was to compare the appropriateness of... |
| Tipo: Journal article |
Palavras-chave: Avocados; Heterogeneity; Multiple comparison; Ripening; Standard deviation. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300002 |
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| Registros recuperados: 9.178 | |
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